The integrated software simplifies data analysis to accelerate response time, including the creation of publication-quality graphs and charts. Capture high-resolution fluorescence and brightfield images, recording data in real-time over hours, days, or weeks. Cells are dynamic in nature, so it is important to have the ability to image in real time to gain access to deeper biological insights. Traditional end-point assays only provide single measurement of cellular events. Live-cell imaging and analysis should be used when studying any area of cell therapeutics.

Advantages of Live-Cell Imaging

If imaged over time, real-time dynamic data can be collected and events that could be missed with endpoint, fixed cell assays are captured. However, fixed (dead) cells cannot provide dynamic insights into biological function and do not represent living systems. Efforts to reduce the cost and complexity of live-cell imaging systems, along with the development of user-friendly software, will help to make these technologies more widely available. This will provide more comprehensive insights into cellular behavior and interactions within complex tissue structures. High-resolution images provide more detail but may require longer exposure times, which can be harmful to cells. These systems use high-definition microscopy to capture detailed, time-lapse images of cellular processes while incorporating features like noise reduction and artifact correction to improve image quality.
More challenges arise when considering the complexity that automation and incubation, which are critical for long-term live-cell imaging, add to the process. Live-cell imaging of T cells engineered to target and kill GFP labelled tumor cells. As research demands grow, high-throughput, automated live-cell analysis helps streamline workflows, reduce errors, and improve data reliability.
This capability is essential for capturing transient events and understanding the temporal dynamics of cellular functions. Fluorescence microscopy allows for precise quantitative measurements of various cellular processes. This results in high-contrast images that clearly delineate different structures within the cell, making it easier to study their interactions and functions. Fluorescence microscopy enhances the visibility of cellular components by using fluorescent dyes or proteins that emit light when excited by specific wavelengths. Long-term live-cell imaging often involves repetitive tasks that can be efficiently managed through automation. Advanced environmental chambers and incubators are essential for creating stable and consistent conditions that support long-term cell culture.

• If imaged over time, real-time dynamic data can be collected and events that could be missed with endpoint, fixed cell assays are captured.
• Discover which live-cell imaging and analysis instrument works best for your lab.
• Robotic systems can handle tasks such as media changes, cell feeding, and image acquisition, ensuring consistency and providing researchers the bandwidth to focus on data analysis and interpretation.
• The ability to make immediate adjustments based on live data reduces the time and resources spent on trial-and-error, leading to more streamlined and effective experimental designs.
• As research demands grow, high-throughput, automated live-cell analysis helps streamline workflows, reduce errors, and improve data reliability.
• See how the Incucyte® can support your research needs, from setting up an assay to analyzing and exporting your data.

These systems typically do not sit within a tissue culture incubator and so are limited in terms of environmental stability and long-term studies. As such, it becomes easier to target internal cellular structures with dyes and antibodies. The fixation process kills the cells, which makes the outside cell membrane more permeable than living cells. While cellular composition is compromised, larger structures such as proteins, organelles and DNA are conserved. Fixed cells have been preserved by goatz casino no deposit bonus a fixation step that “locks” the cell in place.

Incucyte® devices for Cell Health and Proliferation

Quantitative fluorescence imaging enables the measurement of dynamic changes in real-time, offering a deeper understanding of cellular behavior. Derive meaningful data with sensitive, real-time live cell measurements made inside the stable condition of the incubator. Perform real-time, live-cell imaging and analysis of cell health, movement, morphology and function directly f… Make the complex simple with powerful and integrated live-cell imaging and analysis tools that support your entire research team. Easy-to-use software to support all scientists across variety of cell models – turn complex assays into reliable and objective publication-ready data

• By enabling the study of living cells in their natural environment, live-cell imaging offers a more accurate and comprehensive view of biological processes.
• To ensure accurate representation of cellular dynamics, it’s important to optimize acquisition speed, resolution, and time intervals based on the specific biological phenomena being studied.
• The timing of adjunct studies such as analysis of metabolites or secreted proteins in supernatants can also be guided.
• The content of our website is always available in English and partly in other languages.
• Live-cell imaging and analysis also provides the opportunity to make data driven decisions while the experiment is in progress.
• Imaging fixed cells allows high-resolution imaging of sub-cellular structures.

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Environmental Control

Make informed decisions about your cultures, rapidly optimize and improve your workflows, plus study complex live-cell assays to fast track your next discovery! Many technologies such as multi-mode readers and high-content imagers do not have the ability to maintain environmental control, meaning cells are not kept at physiologically relevant conditions. With advancements in modern high-resolution imaging techniques and the synthesis of fluorescent probes, it is now possible to view labeled sub-cellular structures at the nm scale. The Incucyte® Live-Cell Analysis System automatically monitors cells for days, weeks or even months as they sit stationary in the stable tissue culture incubator environment. Get answers to the most common questions about about Sartorius image and analysis solutions including critical information regarding instruments, applications, software, service, and more. Making advanced live-cell imaging technology accessible to all researchers is of critical importance.
Automated systems in live-cell analysis streamline image acquisition and processing, enabling efficient, accurate, and reproducible results. Fluorescence microscopy systems are designed to acquire images at high speeds, enabling researchers to observe rapid changes and dynamic processes in real-time. By tagging specific molecules with fluorescent markers, researchers can track their movement, concentration, and interactions within the cell, providing valuable data for understanding cellular mechanisms. The ability to selectively label and visualize specific molecules provides valuable insights into cellular processes that would otherwise be invisible. Efficient data management systems and advanced analytical tools are essential to handle this influx of information, enabling researchers to extract meaningful insights without being overwhelmed by the data.
The content of our website is always available in English and partly in other languages. See how the Incucyte® can support your research needs, from setting up an assay to analyzing and exporting your data. Incucyte® S3 shares the same highest maximum throughput as the Incucyte® SX5 but supports only the phase channel as well as green and red fluorescence. For the Incucyte® S3, an incubator larger than 200L is recommended.

The Impact of Live-Cell Imaging on Cell Biology

The integration of 3D cell culture further enhances this approach by mimicking the structural complexity of living tissues, offering deeper insights into development, disease, and therapeutic response. Live-cell imaging has become essential for studying the dynamic biology of living cells. Make new discoveries with a wide range of applications  — from complex immune-tumor cell interactions, synaptic activity in neuronal co-cultures, metabolism in cancer cells, and much more.
To ensure accurate representation of cellular dynamics, it's important to optimize acquisition speed, resolution, and time intervals based on the specific biological phenomena being studied. However, over-collection of images can lead to excess data, increasing processing time and storage demands without necessarily enhancing biological insight. The ability to quickly capture images is crucial for studying fast kinetics in cellular activities.

Incucyte® SX5 Live-Cell Analysis System

The integration of AI and machine learning in live-cell imaging will enable quicker and deeper data mining. By enabling the study of living cells in their natural environment, live-cell imaging offers a more accurate and comprehensive view of biological processes. To ensure the best possible image quality, researchers must optimize various settings such as exposure time, focus, and illumination intensity. Optimizing assays for extended kinetic experiments is key to maintaining cell health and ensuring high-quality, reproducible imaging.

Analyze the 3D maturation and 2D growth over time, capturing key biological processes like proliferation and morphological changes. Reduce photobleaching and phototoxicity with patent-pending compact spinning disk technology all while operating within the controlled environment of an incubator The Incucyte® CX3 Live-Cell Analysis System delivers cutting-edge capabilities for 3D cell culture applications. Live-Cell Imaging and Analysis – Real-time, Quantitative and Inside your Incubator Choose your preferred language and we will show you the content in that language, if available.
With the insights gained from Incucyte® assays, you can make informed decisions, quickly optimize workflows, and efficiently study complex live-cell assays to accelerate your next discovery. From proliferation assays to the immunological killing of tumor spheroids, this flexible system allows users to observe and quantify complex biological changes in real-time. These advanced devices allow you to analyze your cells for days, weeks or even months as they sit stationary in the stable environment of your tissue culture incubator Power your research with the Incucyte® platform of real-time live cell analyzers.